5ZTC
Apo structure of TetR family transcription regulator Lmo2088 of Listeria monocytogenes EGDe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.720, 41.960, 71.660 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 36.613 - 1.700 |
| R-factor | 0.1871 |
| Rwork | 0.186 |
| R-free | 0.20540 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.836 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.097 | 0.633 |
| Number of reflections | 55811 | 8969 |
| <I/σ(I)> | 9.81 | 2.28 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 4.5 | 4.45 |
| CC(1/2) | 0.994 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Sodium citrate pH 5.0, 30 % Jeffammine ED2001 pH 7.0 |
