5ZT8
SirB from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-06 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.690, 53.370, 80.430 |
Unit cell angles | 90.00, 105.42, 90.00 |
Refinement procedure
Resolution | 38.766 - 2.000 |
R-factor | 0.1868 |
Rwork | 0.185 |
R-free | 0.21830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zt7 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.766 | 38.766 | 2.100 |
High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
Rmerge | 0.067 | 0.014 | 0.669 |
Rmeas | 0.080 | 0.017 | 0.799 |
Number of reflections | 38718 | 579 | 10174 |
<I/σ(I)> | 14.48 | 61.23 | 1.93 |
Completeness [%] | 99.3 | 96.8 | 98.9 |
Redundancy | 3.448 | 3.634 | 3.307 |
CC(1/2) | 0.998 | 1.000 | 0.741 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.1 M CHES, 20% (w/v) PEG 8000 |