5ZT0

Crystal Structure of Protein Phosphate 1 Complexed with PP1 binding domain of GL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 32
Unit cell lengths	106.530, 106.530, 187.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.792 - 3.320
R-factor	0.20157166675
Rwork	0.200
R-free	0.23315
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mp0
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP (11.0.05)
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.258	3.500
High resolution limit [Å]	3.320	3.320
Rmerge	0.197	1.644
Number of reflections	35147	5089
<I/σ(I)>	6.8	1.2
Completeness [%]	98.9	99.4
Redundancy	4.6	4.6
CC(1/2)		0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	277	0.1M Na/K phosphate, 26%MPD