5ZS9
SufS from Bacillus subtilis in the resting state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 91.900, 91.900, 127.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.213 - 2.800 |
R-factor | 0.1832 |
Rwork | 0.180 |
R-free | 0.23720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j8q |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.213 | 43.213 | 2.900 |
High resolution limit [Å] | 2.800 | 10.000 | 2.800 |
Rmerge | 0.107 | 0.023 | 0.840 |
Rmeas | 0.114 | 0.025 | 0.894 |
Number of reflections | 15771 | 647 | 2930 |
<I/σ(I)> | 20.15 | 76.7 | 2.7 |
Completeness [%] | 100.0 | 98.8 | 100 |
Redundancy | 8.784 | 8.414 | 8.767 |
CC(1/2) | 0.998 | 1.000 | 0.755 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.05M Lithium sulfate, 0.1M Tris-HCl, 50% (v/v) PEG 200 |