5ZS6

Dodecameric structure of a small Heat Shock Protein from Mycobacterium marinum M: Form-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-01-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	I 2 3
Unit cell lengths	91.290, 91.290, 91.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.550 - 2.812
R-factor	0.202519140461
Rwork	0.199
R-free	0.25024
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gme
RMSD bond length	0.005
RMSD bond angle	0.923
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.552	2.960
High resolution limit [Å]	2.810	2.810
Rmerge	0.206	0.627
Rpim	0.045
Number of reflections	3204	466
<I/σ(I)>	12.7	5.1
Completeness [%]	100.0	96.2
Redundancy	21.9	22.3
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.2 M L-Proline, 0.1 M HEPES pH 7.5 and 24 % PEG 1500