5ZRU
Crystal structure of Agl-KA catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.247, 126.423, 80.540 |
Unit cell angles | 90.00, 97.83, 90.00 |
Refinement procedure
Resolution | 19.947 - 1.833 |
R-factor | 0.1426 |
Rwork | 0.141 |
R-free | 0.17120 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.846 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Number of reflections | 98671 | |
<I/σ(I)> | 13.3 | |
Completeness [%] | 99.4 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 10% PEG 6000, 0.1M HEPES(pH8.5), 10mM ZnSO4 |