5ZRT
Crystal structure of human C1ORF123 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.320, 65.350, 95.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.900 - 1.900 |
| R-factor | 0.1778 |
| Rwork | 0.175 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zso |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.037 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.900 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | |
| Number of reflections | 29180 | 10058 |
| <I/σ(I)> | 13.8 | 3 |
| Completeness [%] | 98.1 | 97.2 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.2M magnesium chloride hexahydrate, 0.1M sodium citrate tribasic buffer pH 6.5, 20% polyethylene glycol 3350 |
