5ZRI
M. smegmatis antimutator protein MutT2 in complex with 5m-dCMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.953720 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 65.520, 58.840, 31.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.420 - 1.580 |
R-factor | 0.17834 |
Rwork | 0.177 |
R-free | 0.21116 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zrc |
RMSD bond length | 0.004 |
RMSD bond angle | 0.836 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.420 | 1.660 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.090 | 0.512 |
Number of reflections | 17324 | 2481 |
<I/σ(I)> | 9.1 | 2.3 |
Completeness [%] | 99.7 | 99.2 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 292 | 0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate |