5ZRH
M. smegmatis antimutator protein MutT2 in complex with CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-09-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541790 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.640, 59.550, 31.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.740 - 1.540 |
| R-factor | 0.16899 |
| Rwork | 0.167 |
| R-free | 0.20469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zrc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.294 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.740 | 1.630 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.075 | 0.586 |
| Number of reflections | 18346 | 2429 |
| <I/σ(I)> | 24.6 | 4.6 |
| Completeness [%] | 98.3 | 91.9 |
| Redundancy | 13.1 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 292 | 0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate |
