5ZQM
Crystal structure of human katanin AAA ATPase domain complex with ATPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 65 |
| Unit cell lengths | 68.907, 68.907, 138.698 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.234 - 2.900 |
| R-factor | 0.24927 |
| Rwork | 0.247 |
| R-free | 0.28514 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zql |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.633 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.109 | 0.069 | 0.386 |
| Rmeas | 0.115 | 0.072 | 0.454 |
| Rpim | 0.036 | 0.019 | 0.231 |
| Total number of observations | 64181 | ||
| Number of reflections | 8212 | 855 | 768 |
| <I/σ(I)> | 11 | ||
| Completeness [%] | 98.5 | 99.4 | 91.5 |
| Redundancy | 7.8 | 12.2 | 3.1 |
| CC(1/2) | 0.998 | 0.083 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.4 M Calcium acetate 20% (v/v) PEG 3,350 |
