5ZQA
Crystal Structure of Penicillin-Binding Protein D2 from Listeria monocytogenes in the apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2014-01-17 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.669, 74.582, 74.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.291 - 1.550 |
R-factor | 0.166 |
Rwork | 0.165 |
R-free | 0.18850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3it9 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.010 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.291 | 1.605 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.088 | 0.325 |
Rmeas | 0.097 | |
Number of reflections | 30734 | 2508 |
<I/σ(I)> | 22.6 | |
Completeness [%] | 97.8 | 81.09 |
Redundancy | 5.8 | 3.6 |
CC(1/2) | 0.997 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.2M Potassium chloride, 20%(w/v) Polyethylene glycol 3,350 |