5ZPT
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 10 at 288 K (2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 288 |
Detector technology | CCD |
Collection date | 2015-11-08 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.269, 64.850, 159.119 |
Unit cell angles | 90.00, 117.10, 90.00 |
Refinement procedure
Resolution | 47.630 - 1.902 |
R-factor | 0.1639 |
Rwork | 0.162 |
R-free | 0.19430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.876 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.146 | 0.098 | 0.701 |
Rmeas | 0.170 | 0.115 | 0.821 |
Rpim | 0.087 | 0.059 | 0.424 |
Total number of observations | 526703 | ||
Number of reflections | 138003 | 7163 | 6831 |
<I/σ(I)> | 6.1 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 3.8 | 3.7 | 3.7 |
CC(1/2) | 0.959 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |