5ZPQ
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 9 at 288 K (1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 288 |
Detector technology | CCD |
Collection date | 2015-11-08 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.605, 64.262, 158.870 |
Unit cell angles | 90.00, 117.03, 90.00 |
Refinement procedure
Resolution | 39.673 - 1.849 |
R-factor | 0.1467 |
Rwork | 0.145 |
R-free | 0.17300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.873 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
Rmerge | 0.096 | 0.033 | 0.639 |
Rmeas | 0.113 | 0.040 | 0.766 |
Rpim | 0.060 | 0.021 | 0.418 |
Total number of observations | 521039 | ||
Number of reflections | 148084 | 7638 | 7367 |
<I/σ(I)> | 7.3 | ||
Completeness [%] | 99.9 | 99.5 | 99.7 |
Redundancy | 3.5 | 3.5 | 3.2 |
CC(1/2) | 0.998 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |