5ZPP
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 8 at 288 K (3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Collection date | 2015-12-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.019, 64.746, 158.925 |
| Unit cell angles | 90.00, 117.00, 90.00 |
Refinement procedure
| Resolution | 40.510 - 1.810 |
| R-factor | 0.145 |
| Rwork | 0.144 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.907 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.840 |
| High resolution limit [Å] | 1.810 | 4.910 | 1.810 |
| Rmerge | 0.111 | 0.053 | 0.725 |
| Rmeas | 0.127 | 0.062 | 0.830 |
| Rpim | 0.062 | 0.031 | 0.403 |
| Total number of observations | 657858 | ||
| Number of reflections | 157934 | 8227 | 7780 |
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 99.0 | 99.6 | 98.1 |
| Redundancy | 4.2 | 3.9 | 4.2 |
| CC(1/2) | 0.984 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
