5ZPM
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 7 at 288 K (2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 288 |
Detector technology | CCD |
Collection date | 2015-11-08 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.866, 64.320, 158.903 |
Unit cell angles | 90.00, 116.92, 90.00 |
Refinement procedure
Resolution | 36.675 - 1.650 |
R-factor | 0.1497 |
Rwork | 0.148 |
R-free | 0.17120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.872 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 4.480 | 1.650 |
Rmerge | 0.086 | 0.035 | 0.680 |
Rmeas | 0.101 | 0.042 | 0.800 |
Rpim | 0.053 | 0.022 | 0.418 |
Number of reflections | 208626 | 10744 | 10332 |
<I/σ(I)> | 8.6 | ||
Completeness [%] | 99.9 | 99.6 | 99.9 |
Redundancy | 3.7 | 3.5 | 3.6 |
CC(1/2) | 0.998 | 0.745 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |