5ZPK
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 6 at 288 K (2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Collection date | 2015-11-08 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.731, 63.767, 158.779 |
| Unit cell angles | 90.00, 116.85, 90.00 |
Refinement procedure
| Resolution | 36.634 - 1.650 |
| R-factor | 0.1957 |
| Rwork | 0.195 |
| R-free | 0.21530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.876 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 4.480 | 1.650 |
| Rmerge | 0.082 | 0.030 | 0.765 |
| Rmeas | 0.096 | 0.036 | 0.899 |
| Rpim | 0.050 | 0.019 | 0.467 |
| Total number of observations | 769534 | ||
| Number of reflections | 206892 | 10670 | 10352 |
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 3.7 | 3.5 | 3.7 |
| CC(1/2) | 0.998 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
