5ZPK

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 6 at 288 K (2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	288
Detector technology	CCD
Collection date	2015-11-08
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	192.731, 63.767, 158.779
Unit cell angles	90.00, 116.85, 90.00

Refinement procedure

Resolution	36.634 - 1.650
R-factor	0.1957
Rwork	0.195
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.006
RMSD bond angle	0.876
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.082	0.030	0.765
Rmeas	0.096	0.036	0.899
Rpim	0.050	0.019	0.467
Total number of observations	769534
Number of reflections	206892	10670	10352
<I/σ(I)>	6.2
Completeness [%]	100.0	99.7	100
Redundancy	3.7	3.5	3.7
CC(1/2)		0.998	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES