5ZPG
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH6 at 293K (1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.283, 64.012, 158.812 |
| Unit cell angles | 90.00, 117.04, 90.00 |
Refinement procedure
| Resolution | 38.881 - 1.649 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.868 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
| High resolution limit [Å] | 1.680 | 4.560 | 1.680 |
| Rmerge | 0.110 | 0.075 | 0.676 |
| Rmeas | 0.126 | 0.086 | 0.779 |
| Rpim | 0.061 | 0.041 | 0.384 |
| Total number of observations | 807456 | ||
| Number of reflections | 196443 | 10117 | 9742 |
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 100.0 | 99.8 | 99.9 |
| Redundancy | 4.1 | 3.9 | 4 |
| CC(1/2) | 0.986 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
