5ZPE
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 288 K (2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.558, 64.465, 158.831 |
| Unit cell angles | 90.00, 117.12, 90.00 |
Refinement procedure
| Resolution | 40.411 - 1.689 |
| R-factor | 0.1523 |
| Rwork | 0.151 |
| R-free | 0.17270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.865 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.096 | 0.064 | 0.646 |
| Rmeas | 0.110 | 0.073 | 0.741 |
| Rpim | 0.053 | 0.035 | 0.361 |
| Total number of observations | 780650 | ||
| Number of reflections | 187451 | 9833 | 9081 |
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 97.2 | 98.8 | 95.4 |
| Redundancy | 4.2 | 4 | 4.1 |
| CC(1/2) | 0.989 | 0.807 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
