5ZPA
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 283 K (2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 283 |
Detector technology | CCD |
Collection date | 2015-12-12 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.664, 64.991, 158.851 |
Unit cell angles | 90.00, 117.09, 90.00 |
Refinement procedure
Resolution | 35.355 - 1.689 |
R-factor | 0.1606 |
Rwork | 0.159 |
R-free | 0.18650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 1iu7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.857 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.720 |
High resolution limit [Å] | 1.690 | 4.590 | 1.690 |
Rmerge | 0.158 | 0.094 | 0.732 |
Rmeas | 0.182 | 0.108 | 0.846 |
Rpim | 0.089 | 0.054 | 0.419 |
Total number of observations | 796272 | ||
Number of reflections | 196807 | 10144 | 9772 |
<I/σ(I)> | 9.8 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 4 | 3.9 | 4 |
CC(1/2) | 0.985 | 0.705 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |