5ZP6
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 277 K (2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.849, 64.794, 158.843 |
| Unit cell angles | 90.00, 117.18, 90.00 |
Refinement procedure
| Resolution | 35.324 - 1.688 |
| R-factor | 0.1579 |
| Rwork | 0.157 |
| R-free | 0.17710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.844 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.720 |
| High resolution limit [Å] | 1.690 | 4.590 | 1.690 |
| Rmerge | 0.184 | 0.119 | 0.823 |
| Rmeas | 0.208 | 0.135 | 0.940 |
| Rpim | 0.096 | 0.062 | 0.449 |
| Total number of observations | 827729 | ||
| Number of reflections | 195405 | 10221 | 9604 |
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 97.8 | 99.2 | 96.3 |
| Redundancy | 4.2 | 4 | 4.2 |
| CC(1/2) | 0.980 | 0.711 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
