5ZP3
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 10 at 288 K (1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Collection date | 2015-12-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.811, 64.969, 159.054 |
| Unit cell angles | 90.00, 117.20, 90.00 |
Refinement procedure
| Resolution | 33.274 - 1.782 |
| R-factor | 0.1484 |
| Rwork | 0.147 |
| R-free | 0.17160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.852 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
| Rmerge | 0.085 | 0.050 | 0.566 |
| Rmeas | 0.099 | 0.058 | 0.669 |
| Rpim | 0.051 | 0.030 | 0.353 |
| Total number of observations | 610106 | ||
| Number of reflections | 167742 | 8658 | 8319 |
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 99.9 | 99.7 | 99.5 |
| Redundancy | 3.6 | 3.5 | 3.5 |
| CC(1/2) | 0.986 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
