5ZP1
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 9 at 288 K (1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Collection date | 2015-12-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.665, 64.739, 158.920 |
| Unit cell angles | 90.00, 117.16, 90.00 |
Refinement procedure
| Resolution | 40.489 - 1.669 |
| R-factor | 0.1467 |
| Rwork | 0.146 |
| R-free | 0.16560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.867 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 4.530 | 1.670 |
| Rmerge | 0.070 | 0.027 | 0.653 |
| Rmeas | 0.081 | 0.031 | 0.751 |
| Rpim | 0.039 | 0.016 | 0.367 |
| Total number of observations | 839213 | ||
| Number of reflections | 200673 | 10471 | 9859 |
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 98.7 | 99.5 | 97.4 |
| Redundancy | 4.2 | 3.9 | 4.1 |
| CC(1/2) | 0.999 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
