5ZP1

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 9 at 288 K (1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	288
Detector technology	CCD
Collection date	2015-12-01
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	193.665, 64.739, 158.920
Unit cell angles	90.00, 117.16, 90.00

Refinement procedure

Resolution	40.489 - 1.669
R-factor	0.1467
Rwork	0.146
R-free	0.16560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.006
RMSD bond angle	0.867
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.700
High resolution limit [Å]	1.670	4.530	1.670
Rmerge	0.070	0.027	0.653
Rmeas	0.081	0.031	0.751
Rpim	0.039	0.016	0.367
Total number of observations	839213
Number of reflections	200673	10471	9859
<I/σ(I)>	9.5
Completeness [%]	98.7	99.5	97.4
Redundancy	4.2	3.9	4.1
CC(1/2)		0.999	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES