5ZOO

Crystal structure of histone deacetylase 4 (HDAC4) in complex with a SMRT corepressor SP1 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	131.424, 131.424, 67.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.989 - 1.850
R-factor	0.1562
Rwork	0.155
R-free	0.17530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vqw
RMSD bond length	0.004
RMSD bond angle	0.705
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.565	1.880
High resolution limit [Å]	1.846	1.850
Number of reflections	57039
<I/σ(I)>	12
Completeness [%]	98.4
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	295	PEG 3350, iso-propanol