5ZO4

inactive state of the nuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	I 4 2 2
Unit cell lengths	122.010, 122.010, 148.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.319 - 2.500
R-factor	0.2178
Rwork	0.213
R-free	0.25790
RMSD bond length	0.008
RMSD bond angle	1.013
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2313: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	19752
<I/σ(I)>	60.99
Completeness [%]	100.0
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris-HCl, pH 8.0, 1 M ammonium sulfate