5ZO3

apo form of the nuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	I 4 2 2
Unit cell lengths	121.257, 121.257, 133.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.461 - 1.493
R-factor	0.1562
Rwork	0.156
R-free	0.17420
RMSD bond length	0.006
RMSD bond angle	0.829
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_3092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.470	1.540
High resolution limit [Å]	1.490	1.490
Number of reflections	80479
<I/σ(I)>	50.45
Completeness [%]	99.3
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris-HCl, pH 8.0, 1 M ammonium sulfate