5ZNY

Structure of mDR3_DD-C363G with MBP tag

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	110
Detector technology	PIXEL
Collection date	2017-11-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.977760
Spacegroup name	P 21 21 21
Unit cell lengths	90.320, 145.150, 178.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.780 - 2.740
Rwork	0.210
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1omp
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.790	2.910
High resolution limit [Å]	2.740	2.740
Number of reflections	62248	62030
<I/σ(I)>	18.29
Completeness [%]	99.8
Redundancy	6.44
CC(1/2)	0.999	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	2.2 M ammonium sulfate, 0.1 M MES pH 5.5