5ZNG
The crystal complex of immune receptor RGA5A_S of Pia from rice (Oryzae sativa) with rice blast (Magnaporthe oryzae) effector protein AVR1-CO39
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-17 |
Detector | DECTRIS PILATUS3 R CdTe 300K |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 66.721, 66.721, 108.328 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.405 - 2.189 |
R-factor | 0.1635 |
Rwork | 0.162 |
R-free | 0.19660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2myv |
RMSD bond length | 0.008 |
RMSD bond angle | 0.886 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.470 | 2.268 |
High resolution limit [Å] | 2.108 | 2.189 |
Number of reflections | 215689 | |
<I/σ(I)> | 10.11 | |
Completeness [%] | 1.0 | |
Redundancy | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1.1 M ammonium tartrate dibasic 0.1 M sodium acetate-HCl, pH 4.6 |