5ZN7
Crystal structure of GH31 alpha-xylosidase from a soil metagenome complexed with xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-22 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 173.915, 124.865, 177.716 |
| Unit cell angles | 90.00, 116.98, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.16672 |
| Rwork | 0.165 |
| R-free | 0.19065 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.207 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.244 |
| Number of reflections | 371318 | 15857 |
| <I/σ(I)> | 20.4 | |
| Completeness [%] | 93.8 | 80.5 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 1.6 M lithium sulfate, 100 mM Tris-HCl |
