5ZN6
Crystal structure of GH31 alpha-xylosidase from a soil metagenome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-08 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 173.146, 124.174, 177.453 |
Unit cell angles | 90.00, 116.81, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.17525 |
Rwork | 0.174 |
R-free | 0.19497 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.295 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.067 | 0.252 |
Number of reflections | 561527 | 23616 |
<I/σ(I)> | 15.9 | 2.8 |
Completeness [%] | 91.2 | 77.2 |
Redundancy | 2.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 1.6 M lithium sulfate, 100 mM Tris-HCl |