5ZN4
X-ray structure of protein kinase ck2 alpha subunit H148N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.590, 45.531, 63.276 |
| Unit cell angles | 90.00, 111.99, 90.00 |
Refinement procedure
| Resolution | 33.772 - 1.651 |
| R-factor | 0.1991 |
| Rwork | 0.197 |
| R-free | 0.23860 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.993 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.772 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.039 | 0.336 |
| Number of reflections | 37338 | 3711 |
| <I/σ(I)> | 21.5 | 2.5 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl, 1.2M ammonium sulfate, 5% acetonitrile, 2mM DTT |
