5ZN1
X-ray structure of protein kinase ck2 alpha subunit in D2O
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.641, 45.543, 63.546 |
| Unit cell angles | 90.00, 111.79, 90.00 |
Refinement procedure
| Resolution | 29.500 - 1.050 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.18000 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.463 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.042 | 0.591 |
| Number of reflections | 137800 | |
| <I/σ(I)> | 58.1 | 2.8 |
| Completeness [%] | 94.5 | 90.6 |
| Redundancy | 6.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.1M Tris-HCl, 1.2M ammonium sulfate, 5% acetonitrile, 2mM DTT |
