5ZMZ
Amyloid core of RIP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 29.440, 4.800, 18.940 |
| Unit cell angles | 90.00, 107.97, 90.00 |
Refinement procedure
| Resolution | 18.016 - 1.400 |
| R-factor | 0.24 |
| Rwork | 0.235 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zck |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.184 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (dev_1555) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.016 | 1.520 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 564 | 136 |
| <I/σ(I)> | 22.5 | |
| Completeness [%] | 94.3 | |
| Redundancy | 12.6 | |
| CC(1/2) | 0.976 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M magnesium chloride, 0.1M Sodium HEPES, pH 7.0, 25% (v/v) PEG 200 |
