5ZMY
Crystal structure of a cis-epoxysuccinate hydrolase producing D(-)-tartaric acids
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 135.159, 158.348, 134.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.110 - 1.870 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.19470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zmu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 158.350 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.128 | 0.608 |
| Number of reflections | 234863 | 17249 |
| <I/σ(I)> | 9.9 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M Tris pH 8.5, 0.1 M magnesium formate dihydrate |
