5ZMO
Sulfur binding domain of ScoMcrA complexed with phosphorothioated DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.022, 57.148, 42.320 |
Unit cell angles | 90.00, 101.35, 90.00 |
Refinement procedure
Resolution | 47.650 - 1.690 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.22730 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.888 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.690 | 1.700 |
Number of reflections | 22244 | |
<I/σ(I)> | 11.4 | |
Completeness [%] | 97.0 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 0.1M HEPES, pH 7.5, 0.8M ammonium phosphate monobasic, 0.8M potassium phosphate monobasic, 1% glycerol |