5ZMF

AMPPNP complex of C. reinhardtii ArsA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-21
Detector	RAYONIX MX300HE
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	90.456, 90.456, 306.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.720 - 3.556
R-factor	0.2804
Rwork	0.275
R-free	0.32740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zme
RMSD bond length	0.013
RMSD bond angle	1.747
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8.1_1168)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.720	3.680
High resolution limit [Å]	3.550	3.550
Number of reflections	16090
<I/σ(I)>	29.2
Completeness [%]	99.3
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM Sodium HEPES pH7.5, 20% Glycerol, 10% PEG6000 and 3% 2-Methyl-2,4-pentanediol, 1 mM AMPPNP, 1 mM Magnesium chloride