5ZM9
Crystal structure of hexacoordinated heme protein from anhydrobiotic tardigrade at pH 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2018-02-21 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.312, 56.627, 66.362 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.890 - 2.700 |
R-factor | 0.21969 |
Rwork | 0.217 |
R-free | 0.26679 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.175 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.163 | 0.619 |
Number of reflections | 4057 | |
<I/σ(I)> | 13 | |
Completeness [%] | 100.0 | |
Redundancy | 6.8 | |
CC(1/2) | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG 400, sodium chloride, HEPES |