5ZM7
Crystal structure of ORP1-ORD in complex with cholesterol at 3.4 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 149.253, 149.253, 149.253 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.199 - 3.401 |
R-factor | 0.2782 |
Rwork | 0.277 |
R-free | 0.31230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ic4 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.581 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.401 |
Rmerge | 0.074 | 0.549 |
Rmeas | 0.076 | 0.565 |
Rpim | 0.018 | 0.133 |
Number of reflections | 8299 | 793 |
<I/σ(I)> | 41.67 | 8.11 |
Completeness [%] | 99.8 | 100 |
Redundancy | 17.9 | 18.1 |
CC(1/2) | 0.964 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100mM Sodium acetate, pH 4.6, 2.0M Sodium formate |