5ZM5

Crystal structure of human ORP1-ORD in complex with cholesterol at 2.6 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97961
Spacegroup name	P 31 2 1
Unit cell lengths	135.803, 135.803, 91.154
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.954 - 2.600
R-factor	0.1793
Rwork	0.178
R-free	0.20050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ic4
RMSD bond length	0.012
RMSD bond angle	1.085
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.124	0.617
Rmeas	0.130	0.644
Rpim	0.038	0.183
Number of reflections	30261	1478
<I/σ(I)>	24.18	4.86
Completeness [%]	100.0	100
Redundancy	12.1	12.3
CC(1/2)		0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	100mM Sodium Cacodylate, pH 6.2, 1.2M Sodium Citrate