5ZM0

X-ray structure of animal-like Cryptochrome from Chlamydomonas reinhardtii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.3
Synchrotron site	BESSY
Beamline	14.3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-19
Detector	RAYONIX MX-225
Wavelength(s)	0.8944
Spacegroup name	P 21 21 21
Unit cell lengths	50.210, 65.120, 151.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.760 - 1.600
Rwork	0.153
R-free	0.18810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cvv
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	XSCALE (Jan 26, 2018)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.760	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.275	1.680
Rmeas	0.286	1.777
Number of reflections	66645	6554
<I/σ(I)>	8.5	1.3
Completeness [%]	100.0	100
Redundancy	13.29	10.16
CC(1/2)	0.992	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1
2