5ZLG
Human duodenal cytochrome b (Dcytb) in zinc ion and ascorbate bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-14 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 65.180, 115.570, 48.600 |
Unit cell angles | 90.00, 118.44, 90.00 |
Refinement procedure
Resolution | 43.114 - 2.800 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.25250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o7g |
RMSD bond length | 0.009 |
RMSD bond angle | 1.248 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.114 | 43.114 | 2.970 |
High resolution limit [Å] | 2.800 | 8.370 | 2.800 |
Rmerge | 1.608 | 0.327 | 21.511 |
Rmeas | 0.016 | 0.329 | 21.733 |
Total number of observations | 387956 | ||
Number of reflections | 7824 | 301 | 1266 |
<I/σ(I)> | 8.47 | 37.04 | 1.03 |
Completeness [%] | 99.3 | 98 | 99.7 |
Redundancy | 49.585 | 56.754 | 48.807 |
CC(1/2) | 0.967 | 0.997 | 0.481 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 20%(v/v) Jeffamine ED-2003, 0.1 M HEPES |