5ZLC
Binary complex of human DNA Polymerase Mu with MndGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-31 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 124.719, 124.719, 50.509 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.407 - 2.000 |
R-factor | 0.20346 |
Rwork | 0.202 |
R-free | 0.24060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lzd |
RMSD bond length | 0.009 |
RMSD bond angle | 1.339 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 26362 | 2648 |
<I/σ(I)> | 41.38 | 5.34 |
Completeness [%] | 95.8 | 98.1 |
Redundancy | 7.8 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1 M HEPES pH 7.5, 1.5 M Li2SO4 |