5ZL2
Crystal structure of Bourbon virus envelope glycoprotein at pH8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 3 |
| Unit cell lengths | 102.255, 102.255, 134.472 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.790 - 2.703 |
| R-factor | 0.304332 |
| Rwork | 0.201 |
| R-free | 0.23970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.621 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.186 | |
| Rpim | 0.085 | 0.483 |
| Number of reflections | 42840 | |
| <I/σ(I)> | 8.579 | 1.09 |
| Completeness [%] | 99.4 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.961 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.1M Tris, pH 8.0 and 30%(v/v) polyethylene glycol 400 |
