5ZKX

The postfusion structure of human-infecting Bourbon virus envelope glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97774
Spacegroup name	P 3
Unit cell lengths	101.947, 101.947, 134.506
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.666 - 2.300
R-factor	0.1774
Rwork	0.174
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xea
RMSD bond length	0.004
RMSD bond angle	0.621
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rpim	0.061	0.333
Number of reflections	69252	6728
<I/σ(I)>	12.205	1.477
Completeness [%]	99.6	96.8
Redundancy	7.6	5.1
CC(1/2)	0.998	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M sodium malonate, pH 7.0 and 12%(w/v) polyethylene glycol 3,350