5ZKW
Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with GF2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-07-30 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 116.121, 79.233, 140.571 |
Unit cell angles | 90.00, 100.38, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.860 |
R-factor | 0.16773 |
Rwork | 0.166 |
R-free | 0.19420 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.366 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.890 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 209495 | |
<I/σ(I)> | 20.8 | |
Completeness [%] | 99.2 | |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 10% PEG 3350,0.1 M Sodium malonate (pH 4.2) |