5ZKK
Crystal structure of Phosphoserine phosphatase from Entamoeba histolytica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-08-06 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54178 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 109.172, 109.172, 165.911 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 94.550 - 2.000 |
R-factor | 0.16823 |
Rwork | 0.167 |
R-free | 0.20193 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ij5 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.166 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.550 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 39785 | 2002 |
<I/σ(I)> | 27.6 | 3.4 |
Completeness [%] | 98.7 | 100 |
Redundancy | 14.6 | 13.8 |
CC(1/2) | 0.996 | 0.802 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 289.15 | 0.1 M phosphate citrate buffer, pH 4.2, 4% PEG 300 |