5ZKF
Crystal Structure of N-terminal Domain of Plasmodium vivax p43 in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.181, 86.942, 78.411 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 43.360 - 2.750 |
| R-factor | 0.221 |
| Rwork | 0.215 |
| R-free | 0.27850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.160 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.350 | 2.800 |
| High resolution limit [Å] | 2.748 | 2.750 |
| Number of reflections | 18633 | 844 |
| <I/σ(I)> | 5.4 | 0.9 |
| Completeness [%] | 99.5 | 89.8 |
| Redundancy | 6.4 | 5.3 |
| CC(1/2) | 1.000 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M sodium citrate, 20 % w/v PEG 3350 |
