5ZK4
The structure of DSZS acyltransferase with carrier protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-01-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.324, 96.629, 99.056 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.360 - 2.030 |
| R-factor | 0.18483 |
| Rwork | 0.183 |
| R-free | 0.22236 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sbm |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.095 | 0.942 |
| Number of reflections | 56711 | 4114 |
| <I/σ(I)> | 12.9 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2M ammonium sulfate and 5% isopropanol |
