5ZJU
Crystal structure of in vitro expressed and assembled PCV2 Virus-like Particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL17A |
Synchrotron site | NSRRC |
Beamline | BL17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 194.116, 201.877, 231.281 |
Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.1657 |
Rwork | 0.163 |
R-free | 0.22408 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r0r |
RMSD bond length | 0.019 |
RMSD bond angle | 2.028 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MoRDa |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 432268 | |
<I/σ(I)> | 13.5 | 2.31 |
Completeness [%] | 99.4 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulfate, Citrate |