5ZJT
Structure of AbdB/Exd complex bound to a 'Black14' DNA sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 45.440, 45.630, 66.860 |
Unit cell angles | 99.05, 100.37, 114.18 |
Refinement procedure
Resolution | 63.551 - 2.400 |
R-factor | 0.2367 |
Rwork | 0.232 |
R-free | 0.27590 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.468 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.551 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 16923 | |
<I/σ(I)> | 6.8 | |
Completeness [%] | 93.1 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1M Sodium citrate pH 5.3, 25% PEG 3350 |