5ZJS

Structure of AbdB/Exd complex bound to a 'Blue14' DNA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979180
Spacegroup name	C 1 2 1
Unit cell lengths	77.760, 49.658, 96.774
Unit cell angles	90.00, 109.03, 90.00

Refinement procedure

Resolution	28.143 - 2.896
R-factor	0.2546
Rwork	0.253
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zjq
RMSD bond length	0.004
RMSD bond angle	0.697
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.143	2.999
High resolution limit [Å]	2.896	2.896
Number of reflections	7524
<I/σ(I)>	9.16
Completeness [%]	94.7
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200mM MgCl2, 0.1M Tris pH 5.8, 17.5% PEG 3350, 2.5% glycerol